The transcription factor ABSCISIC ACID INSENSITIVE5 (ABI5) of the abscisic acid (ABA) signaling pathway plays a central role in the inhibition of seed germination. ABI5 is precisely regulated by the core ABA signaling components and multiple other factors. However, the complex regulatory network of ABI5 remains largely unknown.
Abstract ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that plays a key role in the regulation of seed germination and early seedling growth in the presence of ABA and abiotic stresses.
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The phytohormone abscisic acid is a major phytohormone that regulates plant growth and development, including seed dormancy, seed germination, seedling growth, and stomatal aperture, as well as plant responses to various abiotic and biotic stresses, such as drought, salt, and cold stresses and pathogen infection (Mauch-Mani and Mauch, 2005; Fujita et al., 2011; Hauser et al., 2011). Furthermore, we discovered that MED16 may possibly compete with MED25 to interact with the key transcription factor ABA INSENSITIVE 5 (ABI5) to positively regulate ABA signaling. Consistently, med16 and med25 mutants displayed opposite phenotypes in ABA response, cuticle permeability, and differential ABI5‐mediated EM1 and EM6 expression. To functionally analyze the involvement of BIN2 phosphorylation sites in ABI5 activity, we fused the mutant protein ABI5 S41A T35A S36A with HA tag, designated as the HA-ABI5 mutant, and introduced the mutant protein into the abi5-1 background under its native promoter (abi5-1/HA-ABI5 mutant).
To functionally analyze the involvement of BIN2 phosphorylation sites in ABI5 activity, we fused the mutant protein ABI5 S41A T35A S36A with HA tag, designated as the HA-ABI5 mutant, and introduced the mutant protein into the abi5-1 background under its native promoter (abi5-1/HA-ABI5 mutant). Sumoylation of ABI5 by the ArabidopsisSUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling Kenji Miuraa,b,1, Jiyoung Leec,2, Jing Bo Jina,3, Chan Yul Yooa, Tomoko Miuraa, and Paul M. Hasegawaa,1 ABI5 Our initial mapping of ABI5 localized it to the lower arm of chromosome 2, near the phenotypic marker pyrimidine-requiring (py) (Finkelstein, 1994).
Abi5 LLC is a Florida Domestic Limited-Liability Company filed On February 22, 2021. The company's filing status is listed as Active and its File Number is L21000090327. The Registered Agent on file for this company is AB Asset Management LLC and is located at 301 Arthur Godfrey Road, Miami Beach, FL 33140.
Binding Factor3 This review focuses on the role of ubiquitination in. ABA signaling, from ABI5 protein and transcript accumulation is highly regulated by ABA. WT Ws seeds were ABI5 levels were monitored by Western blot analysis.
SUMO (small ubiquitin-related modifier) conjugation (i.e., sumoylation) to protein substrates is a reversible posttranslational modification that regulates signaling by modulating transcription factor activity. This paper presents evidence that the SUMO E3 ligase SIZ1 negatively regulates abscisic acid (ABA) signaling, which is dependent on the bZIP transcripton factor ABI5. Loss-of-function T
Therefore, our study uncovers a new role of ABI5 in vegetative development in plants, and implies a role of ABA signaling in vegetative development in Arabidopsis. 2016-11-01 The target genes of ABI5 enable further adaptations to abiotic stresses.
The transgenic 35S:ABI5‐Flag line was constructed in this study (Fig. S5). CoIP assays revealed that the ABI5‐Flag could be precipitated by JAZ3‐GFP in the double transgenic 35S:JAZ3‐GFP/35S:ABI5‐Flag seedlings, suggesting that JAZ3 interacts with ABI5 in vivo (Fig. 4c).
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In this review, we present the current understanding of the way ABI5 functions as (1) a regulator of abiotic stress responses and (2) an integrator of ABA crosstalk with other phytohormones. The results that are described primarily refer to In this study, we generated a genome‐wide DNA‐binding map of ANAC060, indicating that it directly binds to a G‐box motif. ANAC060 binds to the ABI5 promoter and represses ABI5 transcription, rendering seedling growth insensitive to sugar and ABA. Abscisic acid‐insensitive 5 (ABI5) is an essential and conserved plant basic leucine zipper transcription factor whose level controls seed germination and postgerminative development.
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Sumoylering av MYB30, som fungerar parallellt med den ABI5-medierade Sequence analysis revealed that RAP2 proteins are highly divergent except for the
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As a crucial regulator of ABA signaling, ABSCISIC ACID-INSENSITIVE5 (ABI5) is involved in many aspects of plant growth and development, yet its regulation of anthocyanin biosynthesis has not been elucidated. In this study, we found that MdABI5, the apple homolog of Arabidopsis ABI5, positively regulated ABA-induced anthocyanin biosynthesis.
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Genetic and physiological evidence has demonstrated this key role of ABI5 in this process. Overexpression of ABI5 resulted in enhanced sensitivity to ABA treatment, and the abi5 mutant was insensitive to ABA treatment during both seed germination and vegetative growth (Finkelstein & Lynch, 2000; Lopez‐Molina et al., 2001; Tezuka et al., 2013).
HY5 is a highly conserved central regulator of photo-morphogenesis [ 57 , 58 ] that acts downstream of phytochrome-A and UV RESISTANCE LOCUS8 photoreceptors [ 59 , 60 ]. In this review, we summarized the latest advancement on the function of PTMs involved in the regulation of seed germination, in which the PTMs for ABI5- and DELLA-containing proteins play the key roles. Meanwhile, the studies on PTM-based proteomics during seed germination and the crosstalk of different PTMs are also discussed. abi5-1 seedlings grown at low CO 2 showed lower F v /F m values (Fig. 6C, D). CO 2 assimilation in the mature abi5-1 mutant was reduced under low CO 2 conditions, and could be fully recovered by non-photorespiratory (low O 2) conditions (Fig. 9F, G). Characterization of abi5 mutants in a second legume species, pea (Pisum sativum), confirmed a role for ABI5 in the regulation of longevity, seed degreening, and RFO accumulation, identifying ABI5 as a prominent regulator of late seed maturation in legumes.
ABFs/ABI5. Activation of ABFs/ABI5 transcription factors (TFs) by SnRK2s completes the signal relay and links ABA signaling with ABA-dependent gene activation. Like their activation by phosphorylation, degradation of ABFs/ABI5 TFs by 26S proteasome has also been studied in detail (Table 1 and Figure 2).
4c). We critically review old and recent suggestions of the primary roles played by flavonols, the ancient class of flavonoids already present in bryophytes, in the evolution of terrestrial plants. Our reasoning strongly supports the view that the ABA-flavonol relationship may represent a robust trait of land plants, and might have contributed to their adaptation on land.
2015-09-01 ABI5 is the best-studied key molecule in the ABA signaling pathway with respect to PTMs; therefore, this review could serve as a model to guide post-translational studies of important regulators in other plant hormone signaling pathways. Keywords: (de-)phosphorylation; ABA … 2020-11-07 ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that plays a key role in the regulation of seed germination and early seedling growth in the presence of ABA and abiotic stresses. ABI5 functions in the core ABA signaling, which is composed of PYR/PYL/RCAR receptors, PP2C phosp … 2019-03-01 2021-03-22 Genetic and physiological evidence has demonstrated this key role of ABI5 in this process. Overexpression of ABI5 resulted in enhanced sensitivity to ABA treatment, and the abi5 mutant was insensitive to ABA treatment during both seed germination and vegetative growth (Finkelstein & Lynch, 2000; Lopez‐Molina et al., 2001; Tezuka et al., 2013).